LRRC32 anticorps (PE)

N° du produit ABIN2742630

Cet anticorps anti-LRRC32 est un anticorps Souris Monoclonal détectant LRRC32 dans FACS et ELISA. Adapté pour Humain.

Aperçu rapide pour LRRC32 anticorps (PE) (ABIN2742630)

Antigène: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LRRC32 est conjugé à/à la PE

Application: Flow Cytometry (FACS), ELISA

Clone: ANC8C9

Détail du produit anti-LRRC32 anticorps (PE)

Specificité: Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.

Purification: Protein A purified

Immunogène: Recombinant human GARP.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 10⁵ cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate, and anti-human GARP/PE at dilution factor of 1:50 (10 μg/mL). Cells were washed twice, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 3.9 % sub population of gated cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG2a/PE isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze. Protect from light.

Détails sur LRRC32

Antigène: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Autre désignation: LRRC32

Sujet: Name/Gene ID: LRRC32

Synonyms: LRRC32, D11S833E, GARP, Garpin

ID gène: 2615

Pathways: Activated T Cell Proliferation