alpha Actinin 4 anticorps (N-Term)

N° du produit ABIN2745506

Cet anticorps Lapin Polyclonal détecte spécifiquement alpha Actinin 4 dans WB, IHC et IP. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Porc et Hamster.

Aperçu rapide pour alpha Actinin 4 anticorps (N-Term) (ABIN2745506)

Antigène: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivité: Humain, Souris, Rat, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Actinin 4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-alpha Actinin 4 anticorps

Épitope: AA 27-38, N-Term

Fonction: anti-alpha-Actinin-4, pAb (IG-701)

Specificité: Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.

Réactivité croisée (Details): Does not cross-react with actinin-1, -2 or -3.

Attributs du produit: Polyclonal Antibody. Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3. Source: Rabbit. Applications: IHC, IP, WB. Liquid. In PBS containing 1 mg/mL BSA and 0.01 % sodium azide. alpha-Actinin 4 is an actin-bundling protein of ~100 kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

Purification: Antigen affinity purified.

Pureté: Antigen affinity purified.

Ingrédients: Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500 μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting.

Immunogène: Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.

Information d'application

Indications d'application: Use 5 μL (25 μg) per lane for Western blotting.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: In PBS containing 1 mg/mL BSA and 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur alpha Actinin 4

Antigène: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Autre désignation: alpha-Actinin-4

Sujet:

Alternate Names/Synonyms: F-actin Cross-linking Protein

Product Description: alpha-Actinin 4 is an actin-bundling protein of ~100 kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

UniProt: O43707

Pathways: Proton Transport