Polyglutamate Chain anticorps

N° du produit ABIN2745560

Cet anticorps Lapin Polyclonal détecte spécifiquement dans ICC et WB. Il présente une réactivité envers Toutes les espèces et a été mentionné dans 4+ publications.

Aperçu rapide pour Polyglutamate Chain anticorps (ABIN2745560)

Antigène: Polyglutamate Chain

Reactivité: Toutes les espèces

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Détail du produit

Specificité: Recognizes C-terminally located linear glutamate chains of 4 and more glutamate residues.

Réactivité croisée: Toutes les espèces

Immunogène: Polyglutamate peptide.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Référence

MSaad, Bewersdorf: "Light microscopy of proteins in their ultrastructural context." dans: Nature communications, Vol. 11, Issue 1, pp. 3850, (2020) (PubMed).

Tosetti, Dos Santos Pacheco, Bertiaux, Maco, Bournonville, Hamel, Guichard, Soldati-Favre: "Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii." dans: eLife, Vol. 9, (2020) (PubMed).

Le Guennec, Klena, Gambarotto, Laporte, Tassin, van den Hoek, Erdmann, Schaffer, Kovacik, Borgers, Goldie, Stahlberg, Bornens, Azimzadeh, Engel, Hamel, Guichard: "A helical inner scaffold provides a structural basis for centriole cohesion." dans: Science advances, Vol. 6, Issue 7, pp. eaaz4137, (2020) (PubMed).

Gambarotto, Zwettler, Le Guennec, Schmidt-Cernohorska, Fortun, Borgers, Heine, Schloetel, Reuss, Unser, Boyden, Sauer, Hamel, Guichard: "Imaging cellular ultrastructures using expansion microscopy (U-ExM)." dans: Nature methods, Vol. 16, Issue 1, pp. 71-74, (2019) (PubMed).

Détail du antigène

Antigène: Polyglutamate Chain

Sujet: Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on gamma-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL, Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions.