Profilin anticorps (full length)

N° du produit ABIN2745561

L’anticorps anti-Profilin Polyclonal Lapin est utilisé pour la détection de Profilin dans des échantillons de Humain, Boeuf (Vache), Souris, Porc et Rat. Il a été validé pour WB, IHC et ICC.

Aperçu rapide pour Profilin anticorps (full length) (ABIN2745561)

Antigène: Profilin (PFN)

Reactivité: Humain, Boeuf (Vache), Souris, Porc, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Profilin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Détail du produit anti-Profilin anticorps

Épitope: full length

Fonction: anti-Profilin, pAb (IG-706)

Specificité: Recognizes human, mouse, rat, pig, bovine and marsupial profilin.

Attributs du produit: Polyclonal Antibody. Recognizes human, mouse, rat, pig, bovine and marsupial profilin. Source: Rabbit. Applications: ICC, IHC, WB. Liquid. In PBS containing 1 mg/mL BSA and 0.02 % sodium azide. Profilin (PFN1) is a ubiquitous small (12-15 kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).

Purification: Antigen affinity purified.

Pureté: Antigen affinity purified.

Ingrédients: Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.

Immunogène: Human full-length profilin.

Information d'application

Indications d'application: Use 5 μL (25 μg) per lane for Western blotting of tricine gels (13 % acrylamide) or 15 % Laemmli gels.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur Profilin

Antigène: Profilin (PFN)

Autre désignation: Profilin

Sujet:

Alternate Names/Synonyms: PFN1

Product Description: Profilin (PFN1) is a ubiquitous small (12-15 kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).

UniProt: P07737