The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
Réactivité croisée (Details)
Human, Non-Human Primates
Purification
Purified by protein-A affinity chromatography.
Pureté
> 95 % (by SDS-PAGE)
niveau d'endotoxine
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Functional application: In vitro stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. Flow cytometry: Recommended dilution: 6 μg/mL. Immunohistochemistry (frozen sections): Acetone fixation.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4
Agent conservateur
Azide free
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
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Choi, Wang, Peterson, Letvin, Reimann: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." dans: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).
Congy-Jolivet, Bolzec, Ternant, Ohresser, Watier, Thibault: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." dans: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).
Komano, Nanki, Hayashida, Taniguchi, Miyasaka: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." dans: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, (2007) (PubMed).
Wijngaarden, van Roon, van de Winkel, Bijlsma, Lafeber: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." dans: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).
Metes, Ernst, Chambers, Sulica, Herberman, Morel: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." dans: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).
anticorps Fcgr3, anticorps Fcgr3a, anticorps CD16, anticorps FCGR3, anticorps FCGR3@, anticorps CD16b, anticorps FCG3, anticorps FCR-10, anticorps FCRIII, anticorps FCRIIIb, anticorps FcRIII, anticorps Fc fragment of IgG, low affinity IIa, receptor, anticorps Fc fragment of IgG, low affinity IIIa, receptor (CD16a), anticorps Fc fragment of IgG receptor IIIb, anticorps Fc fragment of IgG receptor IIIa, anticorps Fc receptor, IgG, low affinity III, anticorps Fc-gamma RIII, anticorps Fcgr2a, anticorps FCGR3A, anticorps FCGR3B, anticorps Fcgr3, anticorps FCGR3
Sujet
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII