Cytokeratins anticorps (Biotin)

N° du produit ABIN2749198

Cité dans 4 publications. Cet anticorps anti-Cytokeratins Monoclonal Souris conjugué à Biotin (Clone C-11) (ABIN2749198) détecte spécifiquement Cytokeratins dans ICC, IHC (p), IP, WB et FACS. L’anticorps est réactif avec des échantillons de Mammifères.

Aperçu rapide pour Cytokeratins anticorps (Biotin) (ABIN2749198)

Antigène: Cytokeratins

Reactivité: Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)

Clone: C-11

Détail du produit

Fonction: Anti-Cytokeratins Biotin

Réactivité croisée (Details): Mammalian

Attributs du produit: The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." dans: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." dans: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." dans: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Détail du antigène

Antigène: Cytokeratins

Sujet: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT

ID gène: 35