CHFR anticorps (N-Term)

N° du produit ABIN2774757

Détail du produit anti-CHFR anticorps

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Lapin, Boeuf (Vache), Poisson zèbre (Danio rerio), Cheval, Chien, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHFR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: REWTIGRRRG CDLSFPSNKL VSGDHCRIVV DEKSGQVTLE DTSTSGTVIN

Homologie: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Attributs du produit: This is a rabbit polyclonal antibody against CHFR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 623 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur CHFR

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Autre désignation: CHFR (CHFR Produits)

Synonymes: anticorps RNF116, anticorps RNF196, anticorps CHFR, anticorps fc43f10, anticorps si:dkey-69h6.7, anticorps wu:fc43f10, anticorps rnf116, anticorps rnf196, anticorps 5730484M20Rik, anticorps C230082M18, anticorps checkpoint with forkhead and ring finger domains, anticorps checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase, anticorps checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog, anticorps CHFR, anticorps chfr, anticorps Chfr, anticorps chfr.L

Sujet: CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Alias Symbols: FLJ10796, FLJ33629, RNF116, RNF196
Protein Interaction Partner: USP7, VIM, UBE2D2, AURKA, HLTF, RANBP2, PLK1, PCNA, MCM2, HDAC2, HDAC1, CDK5, CDC20, PARP1, SIN3A, CHFR, UBC13, SUMO1, UBE2I, LRP1, UBE2V1, UBE2N, UBE2D1, TRIM9, RNF125, CBLC, RNF7, RELA, RNF8, HIST2H2BE, HIST2H2AC, UBC, SMARCD1, SMARCB1, SMARCA4, KIF22,
Protein Size: 623

Poids moléculaire: 69 kDa

ID gène: 55743

NCBI Accession: NM_018223, NP_060693

UniProt: Q96EP1