MCM10 anticorps (Middle Region)

N° du produit ABIN2774890

L’anticorps Lapin Polyclonal anti-MCM10 a été validé pour WB. Il convient pour détecter MCM10 dans des échantillons de Humain, Chien, Boeuf (Vache), Lapin, Cheval et Porc.

Aperçu rapide pour MCM10 anticorps (Middle Region) (ABIN2774890)

Antigène: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Reactivité: Humain, Chien, Boeuf (Vache), Lapin, Cheval, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM10 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MCM10 anticorps

Épitope: Middle Region

Séquence: PKLSALAEAK KLAAITKLRA KGQVLTKTNP NSIKKKQKDP QDILEVKERV

Homologie: Cow: 77%, Dog: 86%, Horse: 93%, Human: 100%, Pig: 77%, Rabbit: 86%

Attributs du produit: This is a rabbit polyclonal antibody against MCM10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human MCM10

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 874 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur MCM10

Antigène: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Autre désignation: MCM10

Sujet: This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Alias Symbols: CNA43, MGC126776, PRO2249, DNA43
Protein Interaction Partner: GOLGA2, MCM6, TRIM37, CCND3, ORC3, CDC45, CDC7, UBC, ORC4, ORC2, MCM7, MCM3, MCM2, CDKN2A, CDKN1A, CDK6, CDC6, CDC5L, CCND1, APP, RBX1, VPRBP, CUL4A, DDB1, IKBKG, RPAP1, CEP70, HAUS1, CCDC85B, DPPA2, NINL, ZBTB8B, HOOK2, MCM4, ZBTB43, CEP72, CALCOCO2, KRT
Protein Size: 874

Poids moléculaire: 98 kDa

ID gène: 55388

NCBI Accession: NM_018518, NP_060988

UniProt: B7ZKZ8

Pathways: Mitotic G1-G1/S Phases, DNA Replication