UPF3B anticorps (N-Term)

N° du produit ABIN2776563

Cité dans 1 publication. Cet anticorps anti-UPF3B Polyclonal Lapin (ABIN2776563) détecte spécifiquement UPF3B dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Lapin, Porc et Boeuf (Vache).

Aperçu rapide pour UPF3B anticorps (N-Term) (ABIN2776563)

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Porc, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UPF3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-UPF3B anticorps

Épitope: N-Term

Fonction: UPF3B Antibody - N-terminal region

Séquence: MKEEKEHRPK EKRVTLLTPA GATGSGGGTS GDSSKGEDKQ DRNKEKKEAL

Homologie: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against UPF3B. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human UPF3B

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8115497

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-UPF3B (ABIN2776563)

Mooney, Jimenez-Mateos, Engel, Mooney, Diviney, Venø, Kjems, Farrell, OBrien, Delanty, Henshall: "RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis." dans: Scientific reports, Vol. 7, pp. 41517, (2017) (PubMed).

Détails sur UPF3B

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Autre désignation: UPF3B

Sujet:

Background Information: UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Gene Name: UPF3 regulator of nonsense transcripts homolog B (yeast)

Alternative Symbols: MRX62, MRX82, UPF3X, HUPF3B, MRXS14, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, Upf3p-X

Protein Name: Regulator of nonsense transcripts 3B

Poids moléculaire: 52kDa

ID gène: 65109

NCBI Accession: NP_075386

UniProt: Q9BZI7