Nanog anticorps (Middle Region)

N° du produit ABIN2777175

Cet anticorps Lapin Polyclonal détecte spécifiquement Nanog dans WB. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien, Mouton, Chévre, Cheval, Lapin et Cobaye et a été mentionné dans 4+ publications.

Aperçu rapide pour Nanog anticorps (Middle Region) (ABIN2777175)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Mouton, Chévre, Cheval, Lapin, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Nanog anticorps

Épitope: Middle Region

Séquence: SYKQVKTWFQ NQRMKSKRWQ KNNWPKNSNG VTQKASAPTY PSLYSSYHQG

Homologie: Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 86%, Rat: 92%, Sheep: 86%

Attributs du produit: This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human NANOG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 305 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-Nanog (ABIN2777175)

Kallas, Pook, Maimets, Zimmermann, Maimets: "Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells." dans: PLoS ONE, Vol. 6, Issue 4, pp. e19114, (2011) (PubMed).

Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." dans: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, (2010) (PubMed).

Neganova, Zhang, Atkinson, Lako: "Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells." dans: Oncogene, Vol. 28, Issue 1, pp. 20-30, (2009) (PubMed).

Maimets, Neganova, Armstrong, Lako: "Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells." dans: Oncogene, Vol. 27, Issue 40, pp. 5277-87, (2008) (PubMed).

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG

Sujet: NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305

Poids moléculaire: 34 kDa

ID gène: 79923

NCBI Accession: NM_024865, NP_079141

UniProt: Q6NSW7

Pathways: Stem Cell Maintenance