PCK1 anticorps (Middle Region)

N° du produit ABIN2777602

Il existe 1 publication pour ce produit. L’anticorps anti-PCK1 Polyclonal Lapin est utilisé pour la détection de PCK1 dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Cheval, Porc et Cobaye. Il a été validé pour WB.

Aperçu rapide pour PCK1 anticorps (Middle Region) (ABIN2777602)

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Cheval, Porc, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PCK1 anticorps

Épitope: Middle Region

Fonction: PCK1 Antibody - middle region

Séquence: NGFFGVAPGT SVKTNPNAIK TIQKNTIFTN VAETSDGGVY WEGIDEPLAS

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against PCK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human PCK1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108102

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-PCK1 (ABIN2777602)

Perrone, Mattocks, Plummer, Chittur, Mohney, Vignola, Orentreich, Orentreich: "Genomic and metabolic responses to methionine-restricted and methionine-restricted, cysteine-supplemented diets in Fischer 344 rat inguinal adipose tissue, liver and quadriceps muscle." dans: Journal of nutrigenetics and nutrigenomics, Vol. 5, Issue 3, pp. 132-57, (2012) (PubMed).

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1

Sujet:

Background Information: PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Phosphoenolpyruvate carboxykinase 1 (soluble)

Alternative Symbols: PCKDC, PEPCK1, PEPCKC, PEPCK-C

Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Poids moléculaire: 69 kDa

ID gène: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis