MRPS15 anticorps (N-Term)

N° du produit ABIN2777848

Il existe 1 publication pour ce produit. L’anticorps anti-MRPS15 Polyclonal Lapin est utilisé pour la détection de MRPS15 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Cobaye, Lapin, Cheval et Chien. Il a été validé pour WB et IHC.

Aperçu rapide pour MRPS15 anticorps (N-Term) (ABIN2777848)

Antigène: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Lapin, Cheval, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MRPS15 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-MRPS15 anticorps

Épitope: N-Term

Fonction: MRPS15 Antibody - N-terminal region

Séquence: RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVVK RLLSLEMANK

Homologie: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 83%, Rabbit: 86%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against MRPS15. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPS15

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33299-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-MRPS15 (ABIN2777848)

Davies, Rackham, Shearwood, Hamilton, Narsai, Whelan, Filipovska: "Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation." dans: FEBS letters, Vol. 583, Issue 12, pp. 1853-8, (2009) (PubMed).

Détails sur MRPS15

Antigène: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Autre désignation: MRPS15

Sujet:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

Gene Name: Mitochondrial ribosomal protein S15

Alternative Symbols: DC37, S15mt, RPMS15, MPR-S15

Protein Name: 28S ribosomal protein S15, mitochondrial

Poids moléculaire: 30kDa

ID gène: 64960

NCBI Accession: NP_112570

UniProt: P82914