PHD1 anticorps (Middle Region)

N° du produit ABIN2778438

Cité dans 1 publication. Cet anticorps anti-PHD1 Polyclonal Lapin (ABIN2778438) détecte spécifiquement PHD1 dans WB. L’anticorps est réactif avec des échantillons de Humain et Chien.

Aperçu rapide pour PHD1 anticorps (Middle Region) (ABIN2778438)

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivité: Humain, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHD1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PHD1 anticorps

Épitope: Middle Region

Fonction: EGLN2 Antibody - middle region

Séquence: AVLDGSELSY FGQEGMTEVQ CGKVAFQFQC SSDSTNGTGV QGGQIPELIF

Homologie: Dog: 85%, Human: 100%

Attributs du produit: This is a rabbit polyclonal antibody against EGLN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human EGLN2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39775-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-PHD1 (ABIN2778438)

Tennant, Frezza, MacKenzie, Nguyen, Zheng, Selak, Roberts, Dive, Watson, Aboagye, Gottlieb: "Reactivating HIF prolyl hydroxylases under hypoxia results in metabolic catastrophe and cell death." dans: Oncogene, Vol. 28, Issue 45, pp. 4009-21, (2009) (PubMed).

Détails sur PHD1

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Autre désignation: EGLN2

Sujet:

Background Information: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms. The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Name: Egl nine homolog 2 (C. elegans)

Alternative Symbols: EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1

Protein Name: Egl nine homolog 2

Poids moléculaire: 44kDa

ID gène: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis