MBD3 anticorps (N-Term)

N° du produit ABIN2778641

L’anticorps Lapin Polyclonal anti-MBD3 a été validé pour WB. Il convient pour détecter MBD3 dans des échantillons de Humain, Souris, Rat, Chien, Cobaye, Boeuf (Vache) et Cheval.

Aperçu rapide pour MBD3 anticorps (N-Term) (ABIN2778641)

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Boeuf (Vache), Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MBD3 anticorps

Épitope: N-Term

Séquence: SKMNKSRQRV RYDSSNQVKG KPDLNTALPV RQTASIFKQP VTKITNHPSN

Homologie: Cow: 85%, Dog: 100%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against MBD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD3

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 291 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur MBD3

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Autre désignation: MBD3

Sujet: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: SDCBP, KRT15, ICA1, GOLGA2, CEP76, GATAD2B, RCN3, TRIM54, ZNF277, CEP250, ZBTB16, UBC, RARA, KDM1A, HDAC2, HDAC1, MMS19, HIST3H3, GATAD2A, INO80, MTA2, BAZ1B, SMARCA5, RBBP7, RBBP4, HNRNPM, IGBP1, FUS, EWSR1, DDX3X, DDB1, CHD4, CHD3, TAB2, DNMT1, MTA1, NR
Protein Size: 291

Poids moléculaire: 33 kDa

ID gène: 53615

NCBI Accession: NM_003926, NP_003917

UniProt: O95983

Pathways: Chromatin Binding