AUH anticorps (C-Term)

N° du produit ABIN183940

Cet anticorps Lapin Polyclonal détecte spécifiquement AUH dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Rat, Souris, Chien, Boeuf (Vache), Lapin, Cheval, Cobaye et Saccharomyces cerevisiae.

Aperçu rapide pour AUH anticorps (C-Term) (ABIN183940)

Antigène: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache), Lapin, Cheval, Cobaye, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AUH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-AUH anticorps

Épitope: C-Term

Fonction: AUH Antibody - C-terminal region

Séquence: IGMSLAKELI FSARVLDGKE AKAVGLISHV LEQNQEGDAA YRKALDLARE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%

Attributs du produit: This is a rabbit polyclonal antibody against AUH. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human AUH

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8095298

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur AUH

Antigène: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Autre désignation: AUH

Sujet:

Background Information: AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.

Gene Name: AU RNA binding protein/enoyl-CoA hydratase

Protein Name: Methylglutaconyl-CoA hydratase, mitochondrial

Poids moléculaire: 37kDa

ID gène: 549

NCBI Accession: NP_001689

UniProt: Q13825