RAD17 anticorps (C-Term)

N° du produit ABIN2779300

Cité dans 1 publication. Cet anticorps anti-RAD17 Polyclonal Lapin (ABIN2779300) détecte spécifiquement RAD17 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Porc.

Aperçu rapide pour RAD17 anticorps (C-Term) (ABIN2779300)

Antigène: RAD17

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD17 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-RAD17 anticorps

Épitope: C-Term

Fonction: RAD17 Antibody - C-terminal region

Séquence: PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI IIEDYESDGT

Homologie: Cow: 83%, Dog: 75%, Human: 100%, Mouse: 75%, Pig: 83%, Rat: 75%

Attributs du produit: This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109903

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-RAD17 (ABIN2779300)

Tsao, Geisen, Abraham: "Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." dans: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).

Détails sur RAD17

Antigène: RAD17

Autre désignation: RAD17

Sujet:

Background Information: RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

Gene Name: RAD17 homolog (S. pombe)

Alternative Symbols: CCYC, R24L, RAD24, HRAD17, RAD17SP

Protein Name: Cell cycle checkpoint protein RAD17

Poids moléculaire: 66kDa

ID gène: 5884

NCBI Accession: NP_579919

UniProt: O75943