MBD2 anticorps (N-Term)

N° du produit ABIN2779675

Cet anticorps Lapin Polyclonal détecte spécifiquement MBD2 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

Aperçu rapide pour MBD2 anticorps (N-Term) (ABIN2779675)

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-MBD2 anticorps

Épitope: N-Term

Fonction: MBD2 Antibody - N-terminal region

Séquence: RAHPGGGRCC PEQEEGESAA GGSGAGGDSA IEQGGQGSAL APSPVSGVRR

Homologie: Human: 100%, Mouse: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against MBD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32485-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-MBD2 (ABIN2779675)

Zhao, Wu, Bu, Lu, Liang, Cao, Tong, Lu, Wu, Guo et al.: "Epigenetic silence of ankyrin-repeat-containing, SH3-domain-containing, and proline-rich-region- containing protein 1 (ASPP1) and ASPP2 genes promotes tumor growth in hepatitis B virus-positive ..." dans: Hepatology (Baltimore, Md.), Vol. 51, Issue 1, pp. 142-53, (2009) (PubMed).

Détails sur MBD2

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Autre désignation: MBD2

Sujet:

Background Information: MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Gene Name: Methyl-CpG binding domain protein 2

Alternative Symbols: DMTase, NY-CO-41

Protein Name: Methyl-CpG-binding domain protein 2

Poids moléculaire: 32kDa

ID gène: 8932

NCBI Accession: NP_056647

UniProt: Q9UBB5

Pathways: Chromatin Binding