TRIM32 anticorps (N-Term)

N° du produit ABIN2780865

Cité dans 2 publications. Cet anticorps anti-TRIM32 Polyclonal Lapin (ABIN2780865) détecte spécifiquement TRIM32 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin et Cobaye.

Aperçu rapide pour TRIM32 anticorps (N-Term) (ABIN2780865)

Antigène: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM32 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-TRIM32 anticorps

Épitope: N-Term

Fonction: TRIM32 Antibody - N-terminal region

Séquence: MAAAAASHLN LDALREVLEC PICMESFTEE QLRPKLLHCG HTICRQCLEK

Homologie: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Attributs du produit: This is a rabbit polyclonal antibody against TRIM32. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM32

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38965-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-TRIM32 (ABIN2780865)

Streich, Ronchi, Connick, Haas: "Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism." dans: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8209-21, (2013) (PubMed).

Kudryashova, Wu, Havton, Spencer: "Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component." dans: Human molecular genetics, Vol. 18, Issue 7, pp. 1353-67, (2009) (PubMed).

Détails sur TRIM32

Antigène: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Autre désignation: TRIM32

Sujet:

Background Information: TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Gene Name: Tripartite motif containing 32

Alternative Symbols: HT2A, BBS11, TATIP, LGMD2H, LGMDR8

Protein Name: E3 ubiquitin-protein ligase TRIM32

Poids moléculaire: 72kDa

ID gène: 22954

NCBI Accession: NP_036342

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling