CDC27 anticorps (Middle Region)

N° du produit ABIN2781488

Cet anticorps Lapin Polyclonal détecte spécifiquement CDC27 dans WB. Il présente une réactivité avec des échantillons de Humain, Rat, Chien, Boeuf (Vache), Cheval, Lapin et Cobaye.

Aperçu rapide pour CDC27 anticorps (Middle Region) (ABIN2781488)

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivité: Humain, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC27 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CDC27 anticorps

Épitope: Middle Region

Fonction: CDC27 Antibody - middle region

Séquence: KLKMKFPPKI PNRKTKSKTN KGGITQPNIN DSLEITKLDS SIISEGKIST

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against CDC27. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human CDC27

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43603-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur CDC27

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Autre désignation: CDC27

Sujet:

Background Information: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: Cell division cycle 27 homolog (S. cerevisiae)

Alternative Symbols: APC3, HNUC, NUC2, H-NUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E

Protein Name: Cell division cycle protein 27 homolog

Poids moléculaire: 92kDa

ID gène: 996

NCBI Accession: NP_001247

UniProt: P30260