NCF4 anticorps (N-Term)

N° du produit ABIN2782428

Cet anticorps Lapin Polyclonal détecte spécifiquement NCF4 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Lapin, Cheval, Porc, Cobaye, Boeuf (Vache) et Chien.

Aperçu rapide pour NCF4 anticorps (N-Term) (ABIN2782428)

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivité: Humain, Souris, Rat, Lapin, Cheval, Porc, Cobaye, Boeuf (Vache), Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCF4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NCF4 anticorps

Épitope: N-Term

Fonction: NCF4 Antibody - N-terminal region

Séquence: SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE

Homologie: Cow: 85%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 85%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human NCF4

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106445

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur NCF4

Antigène: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Autre désignation: NCF4

Sujet:

Background Information: NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: Neutrophil cytosolic factor 4, 40 kDa

Alternative Symbols: NCF, CGD3, P40PHOX, SH3PXD4

Protein Name: Neutrophil cytosol factor 4

Poids moléculaire: 39kDa

ID gène: 4689

NCBI Accession: NP_038202

UniProt: A8K4F9