RRM1 anticorps (C-Term)

N° du produit ABIN2782438

Détail du produit anti-RRM1 anticorps

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien, Cheval, Lapin, Cobaye, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RRM1 est non-conjugé

Application: Western Blotting (WB)

Séquence: MHFYGWKQGL KTGMYYLRTR PAANPIQFTL NKEKLKDKEK VSKEEEEKER

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Attributs du produit: This is a rabbit polyclonal antibody against RRM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human RRM1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 792 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-RRM1 (ABIN2782438)

Zhu, Wonganan, Lansakara-P, OMary, Li, Cui: "The effect of the acid-sensitivity of 4-(N)-stearoyl gemcitabine-loaded micelles on drug resistance caused by RRM1 overexpression." dans: Biomaterials, Vol. 34, Issue 9, pp. 2327-39, (2013) (PubMed).

Chung, Sandoval, Sloat, Lansakara-P, Cui: "Stearoyl gemcitabine nanoparticles overcome resistance related to the over-expression of ribonucleotide reductase subunit M1." dans: Journal of controlled release : official journal of the Controlled Release Society, Vol. 157, Issue 1, pp. 132-40, (2012) (PubMed).

Wonganan, Chung, Zhu, Kiguchi, Digiovanni, Cui: "Silencing of ribonucleotide reductase subunit M1 potentiates the antitumor activity of gemcitabine in resistant cancer cells." dans: Cancer biology & therapy, Vol. 13, Issue 10, pp. 908-14, (2012) (PubMed).

Détails sur RRM1

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Autre désignation: RRM1 (RRM1 Produits)

Synonymes: anticorps CG5371, anticorps DmRNR1, anticorps Dmel\\CG5371, anticorps RNR1, anticorps RnrM1, anticorps RntL, anticorps l(2)k06709, anticorps XRRM1, anticorps R1, anticorps RIR1, anticorps RR1, anticorps CHUNP6866, anticorps cb396, anticorps cb838, anticorps r1, anticorps sb:cb548, anticorps wu:fb39b07, anticorps wu:fi14b02, anticorps wu:fk95f07, anticorps ribonucleotide reductase catalytic subunit M1, anticorps Ribonucleoside diphosphate reductase large subunit, anticorps ribonucleoside-diphosphate reductase large subunit, anticorps ribonucleotide reductase M1 L homeolog, anticorps ribonucleotide reductase M1, anticorps ribonucleotide reductase M1 polypeptide, anticorps RRM1, anticorps RnrL, anticorps LOC100472317, anticorps rrm1, anticorps rrm1.L, anticorps Rrm1

Sujet: RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. Its gene may play a role in malignancies and disease that involve this region.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Alias Symbols: R1, RIR1, RR1
Protein Interaction Partner: UBC, RRM1, SUMO2, RNF2, BMI1, PPIB, TNPO1, FBXO6, PPP1CC, TMEM177, DNAJB11, SUCLA2, SGSH, NDUFV1, EEF2, DNASE2, COPS5, CDK2, RRM2, RRM2B, KAT5, LRIF1, YWHAG,
Protein Size: 792

Poids moléculaire: 90 kDa

ID gène: 6240

NCBI Accession: NM_001033, NP_001024

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity