NP (Middle Region) anticorps

N° du produit ABIN2783385

L’anticorps Lapin Polyclonal anti- a été validé pour WB. Il convient pour détecter dans des échantillons de Humain, Boeuf (Vache), Souris, Rat, Chien, Cobaye, Cheval, Lapin, Saccharomyces cerevisiae et Poisson zèbre (Danio rerio). Il y a 1 publication disponible.

Aperçu rapide pour NP (Middle Region) anticorps (ABIN2783385)

Antigène: NP

Reactivité: Humain, Boeuf (Vache), Souris, Rat, Chien, Cobaye, Cheval, Lapin, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Épitope: Middle Region

Séquence: GVDTLVVTNA AGGLNPKFEV GDIMLIRDHI NLPGFSGQNP LRGPNDERFG

Homologie: Cow: 86%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Yeast: 91%, Zebrafish: 79%

Attributs du produit: This is a rabbit polyclonal antibody against NP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human NP

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 289 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Référence

Ghanem, Li, Wing, Schramm: "Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase." dans: Biochemistry, Vol. 47, Issue 8, pp. 2559-64, (2008) (PubMed).

Détail du antigène

Antigène: NP

Classe de substances: Viral Protein

Sujet: Defects in NP are the cause of nucleoside phosphorylase deficiency (NP deficiency). It leads to a severe T-cell immunodeficiency with neurologic disorder in children. The specific function of NP is not yet known.This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC117396, MGC125915, MGC125916, PNP, PRO1837, PUNP, NP
Protein Interaction Partner: PNP, UBC, SUMO2, PPME1, SBDS, PSAT1, GMPS, RAB1A, PSMD5, MTAP, ENO2, HECW2, ITGA4, NADK2, TMEM177, DNAJB11, NDUFV1, GLO1, UBD, CDK2, SIRT7, UCHL5, LRIF1, CCDC90B, RBM48, ZHX1, TFB2M, NCALD, TFAM, TP53, APLP1,
Protein Size: 289

Poids moléculaire: 32 kDa

ID gène: 4860

NCBI Accession: NM_000270, NP_000261

UniProt: P00491