NARF anticorps (Middle Region)

N° du produit ABIN2785739

L’anticorps Lapin Polyclonal anti-NARF a été validé pour WB. Il convient pour détecter NARF dans des échantillons de Humain, Souris, Rat, Cheval, Lapin, Boeuf (Vache), Chien et Cobaye.

Aperçu rapide pour NARF anticorps (Middle Region) (ABIN2785739)

Antigène: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reactivité: Humain, Souris, Rat, Cheval, Lapin, Boeuf (Vache), Chien, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NARF est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NARF anticorps

Épitope: Middle Region

Séquence: FRNIQNMILK LKKGKFPFHF VEVLACAGGC LNGRGQAQTP DGHADKALLR

Homologie: Cow: 92%, Dog: 86%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against NARF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human NARF

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 502 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur NARF

Antigène: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Autre désignation: NARF

Sujet: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Alias Symbols: DKFZp434G0420, FLJ10067, IOP2
Protein Interaction Partner: UBC, LEF1, STAT5A, SHFM1, MMS19, CBX5, APP, LMNA,
Protein Size: 502

Poids moléculaire: 55 kDa

ID gène: 26502

NCBI Accession: NM_031968, NP_114174