GCLM anticorps (Middle Region)

N° du produit ABIN2785805

Cet anticorps Lapin Polyclonal détecte spécifiquement GCLM dans WB et IHC. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Lapin, Chien, Cobaye, Cheval et Poisson zèbre (Danio rerio) et a été mentionné dans 2+ publications.

Aperçu rapide pour GCLM anticorps (Middle Region) (ABIN2785805)

Antigène: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Chien, Cobaye, Cheval, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GCLM anticorps

Épitope: Middle Region

Séquence: KPNSNQVNLA SCCVMPPDLT AFAKQFDIQL LTHNDPKELL SEASFQEALQ

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Attributs du produit: This is a rabbit polyclonal antibody against GCLM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human GCLM

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 274 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-GCLM (ABIN2785805)

Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." dans: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." dans: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Détails sur GCLM

Antigène: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Autre désignation: GCLM

Sujet: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GLCLR
Protein Interaction Partner: UBC, NAGK, GLRX3, GNAI2, GCLC, STOX1, IRF7, CALM1, SORD, CUL3,
Protein Size: 274

Poids moléculaire: 31 kDa

ID gène: 2730

NCBI Accession: NM_002061, NP_002052

UniProt: P48507