ETF1 anticorps (N-Term)

N° du produit ABIN2785859

L’anticorps Lapin Polyclonal anti-ETF1 a été validé pour WB et IHC. Il convient pour détecter ETF1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio), Cobaye, Cheval, Lapin et Saccharomyces cerevisiae.

Aperçu rapide pour ETF1 anticorps (N-Term) (ABIN2785859)

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio), Cobaye, Cheval, Lapin, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ETF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ETF1 anticorps

Épitope: N-Term

Séquence: ISLIIPPKDQ ISRVAKMLAD EFGTASNIKS RVNRLSVLGA ITSVQQRLKL

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against ETF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human ETF1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 437 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur ETF1

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Autre désignation: ETF1

Sujet: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
Alias Symbols: D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1
Protein Interaction Partner: KEAP1, UBC, RTF1, NUDC, VPS26A, TSN, CORO1C, CORO1B, HECW2, ALB, UPF1, LIG4, GSPT1, PPP2CA, GSPT2, PABPC1,
Protein Size: 437

Poids moléculaire: 49 kDa

ID gène: 2107

NCBI Accession: NM_004730, NP_004721

UniProt: P62495