OCT4 anticorps (N-Term)

N° du produit ABIN2792671

Cet anticorps Lapin Polyclonal détecte spécifiquement OCT4 dans WB. Il présente une réactivité envers Humain, Porc, Chien, Lapin et Chévre et a été mentionné dans 3+ publications.

Aperçu rapide pour OCT4 anticorps (N-Term) (ABIN2792671)

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivité: Humain, Porc, Chien, Lapin, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-OCT4 anticorps

Épitope: N-Term

Séquence: PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT

Réactivité croisée: Boeuf (Vache), Chien, Humain, Porc, Lapin

Homologie: Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%

Attributs du produit: This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1

Information d'application

Indications d'application: WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 360 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-OCT4 (ABIN2792671)

Li, Moretto-Zita, Soncin, Wakeland, Wolfe, Leon-Garcia, Pandian, Pizzo, Cui, Nazor, Loring, Crum, Laurent, Parast: "BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." dans: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).

Kaeffer, Legrand, Moyon, Frondas-Chauty, Billard, Guzman-Quevedo, Darmaun, Rozé: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." dans: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).

Suo, Han, Wang, Zhang, Zhao, Zhao, Dai: "Oct4 pseudogenes are transcribed in cancers." dans: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: OCT3/4

Sujet: POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 360

Poids moléculaire: 39 kDa

ID gène: 5460

NCBI Accession: NM_002701, NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance