OCT4 anticorps (N-Term)
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- Antigène Voir toutes OCT4 (POU5F1) Anticorps
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Épitope
- N-Term
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Reactivité
- Humain, Porc, Chien, Lapin, Chévre
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp OCT4 est non-conjugé
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Application
- Western Blotting (WB)
- Séquence
- PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT
- Réactivité croisée
- Boeuf (Vache), Chien, Humain, Porc, Lapin
- Homologie
- Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%
- Attributs du produit
- This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogène
- The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
- Top Product
- Discover our top product POU5F1 Anticorps primaire
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- Indications d'application
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WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Commentaires
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Antigen size: 360 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." dans: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." dans: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).
: "Oct4 pseudogenes are transcribed in cancers." dans: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." dans: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
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- Antigène
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Autre désignation
- OCT3/4 (POU5F1 Produits)
- Synonymes
- anticorps OCT3, anticorps OCT4, anticorps OTF-3, anticorps OTF3, anticorps OTF4, anticorps Oct-3, anticorps Oct-4, anticorps POU5F1, anticorps gp9, anticorps gp-9, anticorps oct4, anticorps pou2, anticorps POU-2, anticorps cb197, anticorps spg/pou2, anticorps chunp6868, anticorps wu:fd18d06, anticorps NF-A3, anticorps Oct-3/4, anticorps Oct3, anticorps Oct3/4, anticorps Oct4, anticorps Otf-3, anticorps Otf-4, anticorps Otf3, anticorps Otf3-rs7, anticorps Otf3g, anticorps Otf4, anticorps POU class 5 homeobox 1, anticorps POU domain, class 5, transcription factor 3, anticorps POU domain, class 5, transcription factor 1, anticorps POU5F1, anticorps pou5f3, anticorps Pou5f1
- Sujet
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POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 360 - Poids moléculaire
- 39 kDa
- ID gène
- 5460
- NCBI Accession
- NM_002701, NP_002692
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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