Fluorescein anticorps

N° du produit ABIN2801850

Détail du produit

Antigène: Fluorescein

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA

Specificité: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Fluorescein conjugated Rabbit IgG and Fluorescein conjugated KLH.

Attributs du produit: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Stérilité: Sterile filtered

Immunogène: Fluorescein conjugated Bovine Serum Albumin (BSA)
Immunogen Type: NativeProtein

Information d'application

Indications d'application: This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] Donkey) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product. 

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 90 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Référence

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." dans: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." dans: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Détail du antigène

Antigène: Fluorescein

Classe de substances: Chemical

Sujet: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC