NPT II anticorps

N° du produit ABIN285052

Détail du produit

Antigène: NPT II

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Immunogène: MPTII antibody was raised in rabbit using purified neomycin phosphotransferase II as the immunogen.

Isotype: IgG

Information d'application

Indications d'application: Use at starting dilution 1:500
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: Lot specific

Buffer: Supplied as an IgG fraction in PBS, pH 7.4 with 0.05 % NaN3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C for short term storage. Aliquot and store at -80 °C for long term storage.

Référence

Fu, Guo, Alfano: "Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin." dans: Journal of bacteriology, Vol. 188, Issue 17, pp. 6060-9, (2006) (PubMed).

Détail du antigène

Antigène: NPT II

Autre désignation: NPTII

Sujet: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.