ALPL anticorps (Center)

N° du produit ABIN2854870

Cet anticorps Lapin Polyclonal détecte spécifiquement ALPL dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris et Mouton et a été mentionné dans 1 publication.

Aperçu rapide pour ALPL anticorps (Center) (ABIN2854870)

Antigène: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivité: Humain, Souris, Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ALPL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ALPL anticorps

Épitope: Center

Réactivité croisée: Souris, Chat, Chien, Rat (Rattus), Boeuf (Vache)

Réactivité croisée (Details): Mouse (91 %), Cat (90 %), Dog (89 %), Rat (91 %), Bovine (89 %)

Attributs du produit: Rabbit polyclonal antibody to alkaline phosphatase (liver/bone/kidney) (alkaline phosphatase, liver/bone/kidney)
Alkaline Phosphatase (Tissue Non-Specific) antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-ALPL (ABIN2854870)

Agustina, Asyifa, Aziz, Hernowo: "The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis." dans: Pathology research international, Vol. 2018, pp. 6346409, (2018) (PubMed).

Détails sur ALPL

Antigène: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Autre désignation: Alkaline Phosphatase (Liver/bone/kidney)

Sujet: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Cellular Localization: Cell membrane, Lipid-anchor , GPI-anchor

Poids moléculaire: 57 kDa

ID gène: 249