ALDOB anticorps

N° du produit ABIN2855024

L’anticorps Lapin Polyclonal anti-ALDOB a été validé pour WB, IF, IHC (p) et ICC. Il convient pour détecter ALDOB dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour ALDOB anticorps (ABIN2855024)

Antigène: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ALDOB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-ALDOB anticorps

Réactivité croisée: Humain, Souris, Rat, Tortue

Attributs du produit: Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)
Aldolase B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.09 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-ALDOB (ABIN2855024)

Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." dans: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

Dawson, Biggar, Storey: "Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68830, (2014) (PubMed).

Détails sur ALDOB

Antigène: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Autre désignation: aldolase, fructose-bisphosphate B

Sujet: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Poids moléculaire: 39 kDa

ID gène: 229

UniProt: P05062