OCT4 anticorps

N° du produit ABIN2855068

Cet anticorps Lapin Polyclonal détecte spécifiquement OCT4 dans WB, IF, ICC, IP, FACS, IHC (p) et IHC (fro). Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour OCT4 anticorps (ABIN2855068)

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Classe de qualité: KO Validated

Détail du produit anti-OCT4 anticorps

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)
Oct4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: mouse ESC , human ESC , NT2D1

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.49 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-OCT4 (ABIN2855068)

Feng, Su, Lin, Lin, Wu, Tung, Li, Shieh, Shiau: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." dans: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).

Lee, Liu, Chang, Nieh, Lin, Jao, Chen, Liu: "Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative." dans: Oncotarget, Vol. 7, Issue 17, pp. 23512-20, (2018) (PubMed).

Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." dans: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

Chen, Huang, Lin: "Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma." dans: International journal of oral science, Vol. 6, Issue 4, pp. 212-8, (2015) (PubMed).

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: POU class 5 homeobox 1

Sujet: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Cellular Localization: Nucleus

Poids moléculaire: 39 kDa

ID gène: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance