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KLF4 anticorps (C-Term)

Name: KLF4 anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN2855075
Price: 765 EUR
Availability: InStock

L’anticorps Lapin Polyclonal anti-KLF4 a été validé pour WB, FACS et IP. Il convient pour détecter KLF4 dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

WB Image Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE antibody diluted at 1:3000 (KLF4 anticorps (C-Term))

IP Image Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody, Western blot analysis was performed using KLF4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (KLF4 anticorps (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE antibody diluted at 1:5000 (KLF4 anticorps (C-Term))

FACS

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (KLF4 anticorps (C-Term))

WB Image HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody , diluted at 1:2000. (KLF4 anticorps (C-Term))

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:3000 (KLF4 anticorps (C-Term))

DDX3 suppresses stemness gene signature. (KLF4 anticorps (C-Term))

8 images

(1 reference)

N° du produit ABIN2855075

Aperçu rapide pour KLF4 anticorps (C-Term) (ABIN2855075)

Antigène

Voir toutes KLF4 Anticorps

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivité

Humain, Souris

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp KLF4 est non-conjugé

Application

Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Épitope
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C-Term

Réactivité croisée

Rat (Rattus), Chat, Porc, Mouton, Macaque rhésus, Boeuf (Vache)

Réactivité croisée (Details)

Rat (100 %), Cat (100 %), Pig (100 %), Sheep (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Attributs du produit

Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))
KLF4 antibody

Purification

Purified by antigen-affinity chromatography.

Immunogène

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Isotype

IgG
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB14263 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivité: Humain WB, IHC, IF, IP Hôte: Lapin Monoclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivité: Humain WB, ELISA, IHC, IF, IP Hôte: Lapin Monoclonal 3A1 unconjugated Recombinant Antibody

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) antibody
KLF4 Reactivité: Humain WB, ELISA, IF Hôte: Souris Monoclonal X1 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody
KLF4 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Chien, Lapin, Cobaye, Poisson zèbre (Danio rerio) WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reactivité: Humain, Souris WB, ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reactivité: Humain WB, ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB17718 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal RB18934 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivité: Humain, Souris WB, IF, FACS Hôte: Souris Monoclonal 56CT5-1-6 unconjugated

Indications d'application

Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Commentaires

Positive Control: A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , human ESC , mouse ESC , HCT116 , HCT116 nuclear extract , *MDM-MB-231

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur

Thimerosal (Merthiolate)

Précaution d'utilisation

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Nishimura, Okamoto, Shibue, Mizuta, Shibayama, Yoshino, Murakami, Yamaguchi, Tanaka, Toida, Igarashi: "KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation." dans: PLoS ONE, Vol. 15, Issue 2, pp. e0229744, (2020) (PubMed).
Antigène

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Autre désignation

KLF4

Sujet

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Cellular Localization: Nucleus

Poids moléculaire

55 kDa

ID gène

9314

Pathways

Peptide Hormone Metabolism, Stem Cell Maintenance