PAX8 anticorps

N° du produit ABIN2855097

Cet anticorps anti-PAX8 est un anticorps Lapin Polyclonal détectant PAX8 dans WB, IHC, IF, IHC (p), IP, ICC et ChIP. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour PAX8 anticorps (ABIN2855097)

Antigène: PAX8 (Paired Box 8 (PAX8))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAX8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Classe de qualité: KO Validated

Détail du produit anti-PAX8 anticorps

Réactivité croisée: Chien, Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to PAX8 (paired box 8)
PAX8 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T nuclear extract , DDDDK-tagged PAX8-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-PAX8 (ABIN2855097)

Shi, Yin, Cai, Yan, Jia, Ma, Zhang, Zhang, Gu, Zhang, Di, Zhuang: "PAX8 regulon in human ovarian cancer links lineage dependency with epigenetic vulnerability to HDAC inhibitors." dans: eLife, Vol. 8, (2020) (PubMed).

Sun, Lu, Wu, Ouyang, Xing, Hou, Shi, Wu: "PTEN-knockdown disrupts the morphology, growth pattern and function of Nthy-Ori 3-1 cells by downregulating PAX8 expression." dans: Oncology letters, Vol. 18, Issue 6, pp. 6732-6740, (2019) (PubMed).

Détails sur PAX8

Antigène: PAX8 (Paired Box 8 (PAX8))

Autre désignation: paired box 8

Sujet: This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Nucleus

Poids moléculaire: 48 kDa

ID gène: 7849

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation