Actin, gamma 1 anticorps (N-Term)

N° du produit ABIN2855174

L’anticorps Lapin Polyclonal anti-Actin, gamma 1 a été validé pour WB, IHC (p) et IHC (fro). Il convient pour détecter Actin, gamma 1 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour Actin, gamma 1 anticorps (N-Term) (ABIN2855174)

Antigène: Actin, gamma 1 (ACTG1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin, gamma 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Actin, gamma 1 anticorps

Épitope: N-Term

Réactivité croisée: Cat, Chien, Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
alpha Sarcomeric Actin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-Actin, gamma 1 (ABIN2855174)

Xie, Perrino: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." dans: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).

Chen, Wang, Wei, Tzeng, Lan, Cheng, Kuo: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." dans: Cancers, Vol. 11, Issue 8, (2019) (PubMed).

Li, Sasse, Bayguinov, Ward, Perrino: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." dans: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).

Détails sur Actin, gamma 1

Antigène: Actin, gamma 1 (ACTG1)

Autre désignation: actin gamma 1

Sujet: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Cellular Localization: Cytoplasm , cytoskeleton

Poids moléculaire: 42 kDa

ID gène: 71

UniProt: P63261

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization