TOP2B anticorps (C-Term)

N° du produit ABIN2855447

L’anticorps Lapin Polyclonal anti-TOP2B a été validé pour WB, IP, IF, ICC et IHC (p). Il convient pour détecter TOP2B dans des échantillons de Humain.

Aperçu rapide pour TOP2B anticorps (C-Term) (ABIN2855447)

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP2B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TOP2B anticorps

Épitope: C-Term

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to Topoisomerase II beta (topoisomerase (DNA) II beta 180 kDa)
Topoisomerase II beta antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Jurkat , K562 , THP-1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur TOP2B

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Autre désignation: DNA topoisomerase II beta

Sujet: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants, however, the second variant has not yet been fully described.

Cellular Localization: Cytoplasm , Nucleus , nucleolus

Poids moléculaire: 183 kDa

ID gène: 7155

UniProt: Q02880