MDC1 anticorps
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- Antigène Voir toutes MDC1 Anticorps
- MDC1 (Mdc1 (MDC1))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp MDC1 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Réactivité croisée
- Humain
- Attributs du produit
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Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)
MDC1 antibody [N2N3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogène
- Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.
- Isotype
- IgG
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- Indications d'application
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Commentaires
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Positive Control: HeLa nucleus
Validation: Orthogonal
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." dans: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).
: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." dans: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).
: "
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Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." dans: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).
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- Antigène
- MDC1 (Mdc1 (MDC1))
- Autre désignation
- mediator of DNA damage checkpoint 1 (MDC1 Produits)
- Synonymes
- anticorps 6820401C03, anticorps AA413496, anticorps Nfbd1, anticorps mKIAA0170, anticorps NFBD1, anticorps mediator of DNA damage checkpoint 1, anticorps mediator of DNA-damage checkpoint 1, anticorps Mdc1, anticorps MDC1
- Sujet
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The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Cellular Localization: Nucleus - Poids moléculaire
- 227 kDa
- ID gène
- 9656
- UniProt
- Q14676
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