POLRMT anticorps (Center)

N° du produit ABIN2856044

L’anticorps Lapin Polyclonal anti-POLRMT a été validé pour WB, IF, ICC, IHC (p) et CUT&RUN. Il convient pour détecter POLRMT dans des échantillons de Humain et Souris. Il y a 3+ publications disponibles.

Aperçu rapide pour POLRMT anticorps (Center) (ABIN2856044)

Antigène: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLRMT est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Détail du produit anti-POLRMT anticorps

Épitope: Center

Attributs du produit: Rabbit Polyclonal antibody to mtRNA polymerase (polymerase (RNA) mitochondrial (DNA directed))
mtRNA polymerase antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: HepG2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.31 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-POLRMT (ABIN2856044)

Kasinathan, Henikoff: "Non-B-Form DNA Is Enriched at Centromeres." dans: Molecular biology and evolution, Vol. 35, Issue 4, pp. 949-962, (2019) (PubMed).

Thakur, Henikoff: "Unexpected conformational variations of the human centromeric chromatin complex." dans: Genes & development, Vol. 32, Issue 1, pp. 20-25, (2018) (PubMed).

Sobek, Dalla Rosa, Pommier, Bornholz, Kalfalah, Zhang, Wiesner, von Kleist-Retzow, Hillebrand, Schaal, Mielke, Christensen, Boege: "Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I." dans: Nucleic acids research, Vol. 41, Issue 21, pp. 9848-57, (2014) (PubMed).

Détails sur POLRMT

Antigène: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Autre désignation: MtRNA Polymerase

Sujet: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

Cellular Localization: Mitochondrion

Poids moléculaire: 139 kDa

ID gène: 5442