POLR3A anticorps (N-Term)

N° du produit ABIN2856286

Cet anticorps Lapin Polyclonal détecte spécifiquement POLR3A dans WB, ICC, IF et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour POLR3A anticorps (N-Term) (ABIN2856286)

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3A est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-POLR3A anticorps

Épitope: N-Term

Réactivité croisée: Souris, Poulet, Xenopus laevis, Boeuf (Vache), Xenopus tropicalis

Réactivité croisée (Details): Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)

Attributs du produit: Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
POLR3A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Commentaires:

Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.49 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur POLR3A

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Autre désignation: POLR3A

Sujet: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Cellular Localization: Nucleus

Poids moléculaire: 156 kDa

ID gène: 11128