Pyruvate Kinase anticorps (Center)

N° du produit ABIN2856815

L’anticorps Lapin Polyclonal anti-Pyruvate Kinase a été validé pour WB, IF, IHC (p) et ICC. Il convient pour détecter Pyruvate Kinase dans des échantillons de Humain et Rat.

Aperçu rapide pour Pyruvate Kinase anticorps (Center) (ABIN2856815)

Antigène: Pyruvate Kinase (PK)

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Pyruvate Kinase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-Pyruvate Kinase anticorps

Épitope: Center

Specificité: This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Attributs du produit: Rabbit Polyclonal antibody to Pyruvate Kinase (liver/RBC) (pyruvate kinase, liver and RBC)
Pyruvate Kinase (liver/RBC) antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , rat liver

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur Pyruvate Kinase

Antigène: Pyruvate Kinase (PK)

Autre désignation: Pyruvate Kinase

Sujet: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 62 kDa

ID gène: 5313