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Pyruvate Kinase anticorps (Center)

PK Reactivité: Humain, Rat WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2856815
  • Antigène Voir toutes Pyruvate Kinase (PK) Anticorps
    Pyruvate Kinase (PK)
    Épitope
    • 3
    • 3
    • 2
    • 2
    Center
    Reactivité
    • 9
    • 8
    • 8
    • 4
    • 3
    Humain, Rat
    Hôte
    • 8
    • 5
    • 4
    Lapin
    Clonalité
    • 17
    Polyclonal
    Conjugué
    • 13
    • 3
    • 1
    Cet anticorp Pyruvate Kinase est non-conjugé
    Application
    • 15
    • 9
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Specificité
    This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.
    Attributs du produit
    Rabbit Polyclonal antibody to Pyruvate Kinase (liver/RBC) (pyruvate kinase, liver and RBC)
    Pyruvate Kinase (liver/RBC) antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogène
    Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
    Isotype
    IgG
  • Indications d'application
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
    Commentaires

    Positive Control: 293T , A431 , HeLa , HepG2 , A375 , rat liver

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Antigène
    Pyruvate Kinase (PK)
    Autre désignation
    Pyruvate Kinase (PK Produits)
    Synonymes
    anticorps CG7070, anticorps Dmel\\CG7070, anticorps PYK, anticorps Pyk, anticorps PYK3b, anticorps PYK3c, anticorps Pyruvate kinase, anticorps PK, anticorps pyruvate kinase, anticorps pyruvate kinase (PK), anticorps Pyruvate kinase (PK), anticorps pyruvate kinase PKM, anticorps testis expressed 45, anticorps PyK, anticorps pykA, anticorps PYK3a|PYK3b|PYK3c, anticorps pyk, anticorps pyk1, anticorps pk, anticorps LOC100534543, anticorps TEX45
    Sujet
    The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

    Poids moléculaire
    62 kDa
    ID gène
    5313
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