Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Immunogène
Neuroketal antibody was raised in goat using neuroketal-Conjugate as the immunogen.
Indications d'application
ELISA: >1:4,000, IHC-P: >1:200, WB: >1:2,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Neat serum, with 0.1 % NaN3 as a preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Antigène
Neuroketals
Autre désignation
Neuroketal
Sujet
Neuroketals and neuroprostanes are a class of compounds that result from the oxidation of docosahexenoic acid (DHA), which is enriched in the brain and retina, especially the synaptic membranes and retina. DHA is a membrane polyunsaturated fatty acid that is especially vulnerable to free radical attack because hydrogen radicals easily remove its double bonds. The DHA is oxidized to isoprostane-like compounds called neuroprostanes, which can dehydrate to form highly reactive A4/J4 neuroprostanes . Neuroprostanes can also undergo rearrangement to form D- and E-ring neuroprostanes. These reactive neuroprostanes are called neuroketals because DHA is so concentrated in the nervous system.