Phosphotyrosine anticorps
Aperçu rapide pour Phosphotyrosine anticorps (ABIN285915)
Antigène
Hôte
Clonalité
Conjugué
Application
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Réactivité croisée (Details)
- Recognize proteins and peptides phosphorylated on tyrosine residues. Does not cross - react with phosphoserine and threonine.
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Purification
- Affinity purified
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Immunogène
- Phosphotyrosine antibody was raised in rabbit using Iodoacetylphosphotyrosine-KLH conjugate as the immunogen.
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Indications d'application
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ELISA: 1:1,000, IHC: 1:50, IP: 10 µg/500 µg protein sample, WB: 1:250
Optimal conditions should be determined by the investigator. -
Restrictions
- For Research Use only
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Concentration
- Lot specific
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Buffer
- Tris-acetate buffer, pH 7-7.5, with 0.05 % NaN3.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Conseil sur la manipulation
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20 °C.
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- Phosphotyrosine
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Classe de substances
- Amino Acid
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Sujet
- Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phosphotyrosine which is considered as a key step in the regulation of enzymatic activities such as signal transduction.
Antigène
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