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VRN2 anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement VRN2 dans ELISA et WB. Il présente une réactivité envers Wheat.
N° du produit ABIN7477853

Aperçu rapide pour VRN2 anticorps (ABIN7477853)

Antigène

VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Reactivité

Wheat

Hôte

Souris

Clonalité

Monoclonal

Application

ELISA, Western Blotting (WB)
  • Réactivité croisée (Details)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    Homologie

    Triticum monococcum

    Attributs du produit

    Expected / apparent Molecular Weight of the Antigene: 23.7
    VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 andZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2antibody was able to differentiate VRN-2 from the ZCCT2 protein.

    Purification

    affinity purified

    Immunogène

    KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
  • Indications d'application

    1: 1000 (WB), 1: 10 000 (ELISA)

    Commentaires

    there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Conseil sur la manipulation

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Stock

    -20 °C

    Stockage commentaire

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Antigène

    VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

    Autre désignation

    VRN2

    Sujet

    Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

    Poids moléculaire

    23.7
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