VRN2 anticorps

N° du produit ABIN7477853

Cet anticorps Souris Monoclonal détecte spécifiquement VRN2 dans ELISA et WB. Il présente une réactivité avec des échantillons de Triticum aestivum.

Aperçu rapide pour VRN2 anticorps (ABIN7477853)

Antigène: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Reactivité: Triticum aestivum

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Western Blotting (WB)

Détail du produit anti-VRN2 anticorps

Fonction: Anti-VRN2 | Vernalization protein 2

Réactivité croisée (Details): Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Triticum monococcum

Purification: Protein G purified

Immunogène: KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Information d'application

Indications d'application: 1 : 10 000 (ELISA), 1 : 1000 (WB)

Commentaires:

there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Total IgG. Protein G purified purified from Cell culture supernatant.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détails sur VRN2

Antigène: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Autre désignation: VRN2

Sujet:

Background: Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Additional information: VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Poids moléculaire: 23.7 kDa