Suitable use in ELISA (1: 20 - 1: 200) and Western blot. A starting range of 1: 10-1: 50 for immunohistochemistry has been reported. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL (OD 280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2, containing 0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
HCV NS3
(Hepatitis C Virus NS3 (HCV NS3))
Autre désignation
HCV NS3
Classe de substances
Viral Protein
Sujet
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.Synonyms: Hepatitis C Virus Serine proteiase NS3