PAPPA anticorps
Aperçu rapide pour PAPPA anticorps (ABIN2877949)
Antigène
Voir toutes PAPPA AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Recognizes native Pregnancy-associated plasma protein A (human, PAPP-A) Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of this product is different from those of clones 6F172 and 6F173. The epitope is located on the PAPP-A subunit of the heterotetramer. Reactivity: In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. This product detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.
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Purification
- Protein A/G purified
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Immunogène
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Purified human PAPP-A.
Type of Immunogen: Purified protein -
Isotype
- IgG2b kappa
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Indications d'application
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Approved: ELISA (1:8000), IHC, WB
Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. Western Blot: Detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.2, 15 mM sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
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Autre désignation
- PAPPA / PAPP-A
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Sujet
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Name/Gene ID: PAPPA
Subfamily: Metallopeptidase M43
Family: Protease
Synonyms: PAPPA, DIPLA1, IGF-dependent IGFBP-4 protease, PAPPA1, PAPA, PAPP-A, Pappalysin-1, ASBABP2, IGFBP-4ase -
ID gène
- 5069
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UniProt
- Q13219
Antigène
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