Protein A anticorps (Biotin)

N° du produit ABIN288033

Cet anticorps anti-Protein A est un anticorps Polyclonal produit chez le Chèvre, détectant Protein A dans ELISA, WB et IHC. Convient pour Staphylococcus aureus.

Aperçu rapide pour Protein A anticorps (Biotin) (ABIN288033)

Antigène: Protein A

Reactivité: Staphylococcus aureus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein A est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Protein A anticorps (Biotin)

Purification: Protein A affinity chromatography

Immunogène: Protein A antibody (biotin) was raised in goat using Protein A [Staphylococcus aureus] as the immunogen.

Information d'application

Indications d'application: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: IgG fraction supplied as a liquid in 20 mM Potassium Phosphate and 150 M NaCl, pH 7.2, with 10 g/mL BSA (IgG and Protease free) as a stabilizer, and 0.01 % NaN3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Détails sur Protein A

Antigène: Protein A

Sujet: Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain.