Vitronectin anticorps

N° du produit ABIN2887824

Détail du produit anti-Vitronectin anticorps

Antigène: Vitronectin (VTN)

Reactivité: Humain, Boeuf (Vache), Chien, Chévre, Cheval, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vitronectin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

Purification: Affinity purified

Immunogène: Human vitronectin purified from plasma by heparin-affinity chromatography.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (1:30000), IHC, IHC-P (2.5 μg/mL), WB (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Vitronectin

Antigène: Vitronectin (VTN)

Autre désignation: VTN / Vitronectin (VTN Produits)

Synonymes: anticorps VTN, anticorps DKFZp470F0511, anticorps LOC100228142, anticorps vtn, anticorps zgc:112277, anticorps V75, anticorps VN, anticorps VNT, anticorps AI256434, anticorps Vn, anticorps Aa1018, anticorps vitronectin, anticorps vitronectin a, anticorps vitronectin b, anticorps vitronectin L homeolog, anticorps VTN, anticorps vtn, anticorps vtna, anticorps vtnb, anticorps CpipJ_CPIJ016691, anticorps vtn.L, anticorps Vtn

Sujet: Name/Gene ID: VTN
Family: Matrix Protein

Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

ID gène: 7448

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration