GFP anticorps

N° du produit ABIN289429

Cet anticorps Chèvre Polyclonal détecte spécifiquement GFP dans WB, ELISA et IHC. Il présente une réactivité avec des échantillons de Aequorea victoria. Il a été cité dans 2+ publications.

Aperçu rapide pour GFP anticorps (ABIN289429)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-GFP anticorps

Réactivité croisée (Details): Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml

Purification: Affinity chromatography purified

Stérilité: Filtered

Immunogène: GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

Information d'application

Indications d'application: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

Références pour anticorps anti-GFP (ABIN289429)

Buser, Drubin: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." dans: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).

Buser, McDonald: "Correlative GFP-immunoelectron microscopy in yeast." dans: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.