Latexin anticorps (AA 2-222)

N° du produit ABIN291079

Cet anticorps Mouton Polyclonal détecte spécifiquement Latexin dans WB, ELISA, IP et Neut. Il présente une réactivité envers Souris.

Aperçu rapide pour Latexin anticorps (AA 2-222) (ABIN291079)

Antigène: Latexin (LXN)

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Latexin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Détail du produit anti-Latexin anticorps

Épitope: AA 2-222

Specificité: Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40 % cross-reactivity with rhLatexin.

Purification: Affinity purified

Immunogène: E. coli-derived rmLatexin (aa2-222).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells. Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50 % of the proteolytic activity was restored by the antibody at 4.4 μg/mL (IC50) under conditions in which 0.1 μg/mL of the inhibitor, 0.1 μg/mL of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 Molar ratio of the antibody to the inhibitor. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur Latexin

Antigène: Latexin (LXN)

Autre désignation: Latexin / MUM

Sujet: Name/Gene ID: LXN

Synonyms: LXN, ECI, MUM, Protein MUM, TCI, Latexin

ID gène: 56925

UniProt: Q9BS40